Nucleosome Positioning and Transcription
This extract was created in the absence of an abstract.
Excerpt
In 1977, the topic of the Cold Spring Harbor Symposium was chromatin. At that time, the burgeoning information about nucleosomes and the repetitive packaging of chromosomal DNA highlighted the meeting. Functional implications of this organization of chromatin were of concern to several of the meeting participants. One group asked whether specific associations of DNA sequences with histone octamers in nucleosomes might affect gene expression (Prunell and Kornberg 1978). Others addressed the question of whether histones might have a role in limiting the access of other proteins to DNA. A working hypothesis developed over time that histones could have evolved to package DNA to fit into the eukaryotic nucleus in a nearly transparent fashion, having little or no effect on the information in DNA that was available to nucleoplasmic proteins. This hypothesis has engaged our laboratory for the last 6 years. We have focused on the problem of nucleosome positioning and...
- *
↵* Present address: Department of Biochemistry and Molecular Biology, M. D. Anderson Cancer Center, University of Texas, Houston, Texas 77030
- †
↵† Present address: Molecular Genetics Program, Wadsworth Center for Labs and Research, Empire State Plaza, P.O. Box 509, Albany, New York 12201
- ‡
↵‡ Department of Chemistry and Biochemistry, University of Colorado, Campus Box 215, Boulder, Colorado 80309
- §
↵§ Laboratory of Analytical Chemistry, Tokyo College of Pharmacy, Hachioji, Tokyo 192-03 Japan.
