THE PREPARATION AND ASSAY OF MAMMOTROPIN1
This extract was created in the absence of an abstract.
Excerpt
In this report, consideration is given to one of the methods used in preparing mammotropic hormone, and to some of the assay procedures followed by us. No attempt is made to review or correlate the literature of the field.
Preparation and Extraction Procedure
Since learning that undissected ovine pituitaries yielded as much mammotropin as an equal weight of dissected bovine anterior lobes, the former have been our source material. The glands are frozen between 5 and 10 hours after removal from the sheep, and thawed as needed. No attempt is made to dissect the anterior lobes free from the extraneous material, and since on the one occasion when ovine posterior lobes with their stalks were tested, they yielded about as much mammotropin and adrenocorticotropin as an equal weight of anterior lobes, there seemed but little excuse for excluding them.
Listed below are the ten main steps of one of The
Footnotes
- 1
↵1 The mammary-stimulating, or mammotropic hormone of the anterior pituitary, lactogenic hormone, prolactin, galactin.
