Expression of Flagellar Protein Genes during Flagellar Regeneration in Chlamydomonas
This extract was created in the absence of an abstract.
Excerpt
The growth of cilia or flagella in several different organisms has provided model systems that can be used to investigate the control of synthesis of specific proteins and their assembly into a cell organelle (Rosenbaum and Child 1967; Rosenbaum and Carlson 1969; Rosenbaum et al. 1969; Stephens 1972; Fulton and Kowit 1975). Using cycloheximide to inhibit protein synthesis, it was shown that the regeneration of Chlamydomonas flagella (Rosenbaum et al. 1969) or Tetrahymena cilia (Rosenbaum and Carlson 1969) is dependent on new protein synthesis. In vivo pulse-labeling and one-dimensional SDS-gel analysis of the labeled proteins showed the appearance of newly synthesized ciliary or flagellar proteins in the cytoplasm after deflagellation or deciliation (Weeks et al. 1977; Lefebvre et al. 1978; Guttman and Gorovsky 1979). Reflecting this increased synthesis of tubulin in the cytoplasm, polysomes isolated from regenerating cells contain levels of tubulin mRNA that are higher than those in nonregenerating...
