Characterization of Virus-specified Proteins Present in NRK Cells Infected with a Temperature-sensitive Transformation Mutant of Moloney Murine Sarcoma Virus
This extract was created in the absence of an abstract.
Excerpt
The nondefective mammalian type-C retrovirus genome contains a single-stranded polyadenylated RNA molecule about 9–10 kb long, which consists of at least three genetic regions (Baltimore 1975). These include the gag gene, which is located near the 5′ terminus of the viral genome and which encodes the internal structural proteins of the virus (Jamjoom et al. 1977; Murphy and Arlinghaus 1978), the pol gene, which codes for the viral reverse transcriptase (Kopchick et al. 1978, 1979; Murphy et al. 1978), and the env gene, which is located near the 3′ terminus of the genome (Rothenberg et al. 1978) and which encodes the proteins of the viral envelope (Karshin et al. 1977). Viruses of this group replicate in, but cause no morphological alteration of, cells in culture; they induce neoplastic diseases in vivo only after extended latent periods (Aaronson and Stephenson 1976).
Passage of such nondefective retroviruses in animals has resulted in...
