Hormonal Modulation of α 2u Globulin mRNA: Sequence Measurements Using a Specific cDNA Probe
This extract was created in the absence of an abstract.
Excerpt
α 2u Globulin is a protein of m.w. 20,000, which is synthesized in the liver of mature male rats, secreted into the serum, and excreted in the urine. This protein represents approximately 1% of hepatic protein synthesis in an adult male rat, and no detectable α 2u globulin synthesis occurs in females (Sippel et al. 1976). Our interest in this protein is based on the fact that its rate of hepatic biosynthesis is under multihormonal control: androgens, glucocorticoids, thyroid hormones, and pituitary growth hormone are necessary to maintain normal levels of α 2u globulin synthesis in male rats, and estrogens repress the synthesis of this protein (Sippel et al. 1975; Kurtz et al. 1976a,b). α 2u Globulin can be induced in female rats by ovariectomy and androgen administration (Sippel et al. 1975). Using a wheat-germ cell-free translation system, we have quantitated the level of translationally functional α 2u globulin Mrna...
