Functional and Structural Characterization of Immunoglobulin on Murine B Lymphocytes
This extract was created in the absence of an abstract.
Excerpt
There is no doubt that immunoglobulin is the receptor for antigen on B lymphocytes, but there is certainly some confusion regarding CH gene expression at the level of the lymphocyte surface. There are three very clear reasons for this confusion: First, much of the data comes from immunofluorescence, a technique plagued with technical problems, e.g., material binding nonspecifically or through Fc receptors. What is clear, however, is that the number of B lymphocytes recorded positive for IgG and IgA steadily decrease to a very low number (1–3%) as more care is taken to avoid these problems (Jones et al. 1973; Kurnick and Grey 1975; Lobo et al. 1975; Winchester et al. 1975). Second, particularly in the mouse, when surface immunoglobulin is characterized by external labeling and immunochemical procedures, there is little, if any, IgG or IgA detected (Vitetta et al. 1971; Marchalonis et al. 1972; Abney and Parkhouse 1974). Third,...
