Crystal Structure of an Immunoglobulin Molecule by X-Ray Diffraction and Electron Microscopy
This extract was created in the absence of an abstract.
Excerpt
We have previously reported an X-ray diffraction investigation of the three-dimensional structure at 6 Å resolution of a human γGl immunoglobulin molecule, Dob, (Sarma et al., 1971). We present here the results of a new electron-density map calculated using phases obtained with two additional derivatives. We also present the results of an electron microscopic investigation (Labaw and Davies, 1971) of the immunoglobulin crystals which are in complete accord with the X-ray results.
EXPERIMENTAL METHODS
X-ray diffraction. The details of the crystallization of the Dob immunoglobulin, preparation of heavy atom derivatives and the method of data collection have been described earlier (Sarma et al., 1971). The data for the K2PtCl6 derivative which was used for phasing the previous map were not entirely satisfactory and, in addition, we later had difficulty in reproducing this derivative. Consequently we have rejected these data from the present phase calculations. Two new derivatives, K2PtCl4 and Baker...
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↵* Present address: Department of Biochemistry, State University of New York at Stony Brook, Stony Brook, New York.
